Anti-Human ITGAV Recombinant Antibody (Abituzumab)
CAT#: TAB-H01
Recombinant Humanized (from mouse) antibody to Human ITGAV. Abituzumab (EMD 525797)is a humanised monoclonal antibody targeting integrin αν heterodimers.
Published Data
Gene Expression
Tested Data
Agonist
Figure 4 DI17E6 promotes detachment of prostate cancer cells from vitronectin.
Prostate cancer cell lines (LNCaP, C4-2B, ARCaP, PC3, DU145 and VCaP cells) were seeded on 96-well plates coated with vitronectin.
Jiang, Y. , Dai, J. , Yao, Z. , Shelley, G. , & Keller, E. T. . (2017). Abituzumab targeting of αv-class integrins inhibits prostate cancer progression. molecular cancer research mcr, 15(7), molcanres.0447.2016.
Inhib
Figure 5 DI17E6 inhibits adhesion of prostate cancer cells on vitronectin.
Prostate cancer cell lines (LNCaP, C4-2B, ARCaP, PC3, DU145 and VCaP cells) were seeded on the 96-well plates pre-coated with fibronectin.
Jiang, Y. , Dai, J. , Yao, Z. , Shelley, G. , & Keller, E. T. . (2017). Abituzumab targeting of αv-class integrins inhibits prostate cancer progression. molecular cancer research mcr, 15(7), molcanres.0447.2016.
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4856/if_selected.jpg
Subcellular Location
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4856/ihc_selected.jpg
Subcellular Location
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong membranous positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2499/ihc_selected.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4856/10_E3_1_blue_red_green.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4856/15_E3_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2499/6125_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2499/6125_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2499/6125_A_9_4.jpg
Normal Tissue
Figure 9 Placenta
Cytotrophoblasts
Staining: High
Intensity: Strong
Quantity:>75%
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Syncytiotrophoblasts - microvilli
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2499/6125_A_2_7.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (RT4, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000138448-ITGAV
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SDS-PAGE
Figure 1 Anti-Human ITGAV Recombinant Antibody (TAB-H01) in SDS-PAGE
SDS-PAGE analysis of TAB-H01 in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180 kDa.
SEC-HPLC
Figure 2 Anti-Human ITGAV Recombinant Antibody (TAB-H01) in SEC-HPLC
SEC-HPLC analysis of TAB-H01 shows that the purity is more than 95%.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
WB
Figure 3 Anti-Human ITGAV Recombinant Antibody (TAB-H01) in WB
Western blot analysis of TAB-H01 was performed by loading human Integrin alpha V beta 3 protein (His tag) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-H01 and HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Chemiluminescent detection was performed.
Lane 1: Reduced antigen (0.8 μg)
DB
Figure 4 Anti-Human ITGAV Recombinant Antibody (TAB-H01) in DB
Dot Blot analysis of TAB-H01 was performed by coating with human Integrin alpha V beta 3 protein (His tag).
TAB-H01 incubation concentration: 2 μg/mL.
HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000)
ELISA
Figure 5 Anti-Human ITGAV Recombinant Antibody (TAB-H01) in ELISA
ELISA analysis of TAB-H01 was performed by coating with human Integrin alpha V beta 3 protein (His tag). Then blocked with BSA and incubated with TAB-H01. The HRP conjugated Anti-Human IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG2 - kappa
- Specificity
- ITGAV (integrin alpha V, CD51) [Homo sapiens]
- Species Reactivity
- Human
- Applications
- Inhib, Agonist
- CAS
- 1105038-73-0
- Generic Name
- abituzumab
- Related Disease
- Colorectal cancers, metastatic
Product Property
- Purity
- Purity >95% by SDS-PAGE.
Applications
- Application Notes
- The Human ITGAV antibody has been reported in applications of Inhibition, Agonist.
Target
- Alternative Names
- abituzumab;1105038-73-0;EMD525797;EMD 525797;DI-17E6;DI17E6;ITGAV;integrin, alpha V;antigen identified by monoclonal L230 , integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) , MSK8, vitronectin receptor , VNRA, VTNR;integrin alpha-
- Gene ID
- 3685
- UniProt ID
- P06756
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(Creative Biolabs Cat# TAB-H01, RRID: AB_3112021)
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Biosimilar Overview
Please refer to Abituzumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Abituzumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
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See other products for "Abituzumab"
Afuco™ Anti-ITGAV ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-H01)This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant Humanized (from mouse) antibody to Human ITGAV. It is a humanized monoclonal antibody targeting integrin αν heterodimers.
DrugMonitor™ Anti-Abituzumab Antibody (VS-1224-YC10)Abituzumab has been evaluated in trials investigating treatments for metastatic prostate cancer. The DrugMonitor™ Anti-Abituzumab Antibody (VS-1224-YC10) is an anti-drug antibody (ADA) against Abituzumab. This drug-based antibody is raised in mice immunized with the Abituzumab. The anti-Abituzumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Abituzumab in samples.
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